Fig. 8.
Infusion of ANG II is not associated with changes in renal ANG II type 1A (AT1A) receptor or angiotensin-converting enzyme (ACE)2 expression in Cyp1b1+/+ and Cyp1b1−/− mice but decreased ANG II type 2 (AT2) receptor and ACE2 expression in Cyp1b1−/− mice and Mas receptor expression in Cyp1b1+/+ and Cyp1b1−/− female mice. Cyp1b1+/+ and Cyp1b1−/− mice were infused with vehicle or ANG II for 2 wk. A–E: renal tissue was collected to measure mRNA expression of AT1A receptor (A), AT2 receptor (B), ACE (C), ACE2 (D), and Mas receptor (E), as described in materials and methods. mRNA gene expression was normalized against β-actin, which was used as a housekeeping gene, and calculated on the basis of the ΔΔCt method (where Ct is threshold cycle). Data are expressed as means ± SE; n = 3 for all experiments. *P < 0.05, ANG II vs. the corresponding value with vehicle; †P < 0.05, ANG II-infused Cyp1b1−/− mice vs. ANG II-infused Cyp1b1+/+ mice.