Table 2.

Renal dysfunction caused by infusion of ANG II is observed in Cyp1b1^−/− but not Cyp1b1^+/+ female mice

	Cyp1b1+/+ Mice		Cyp1b1−/− Mice
Parameter	Vehicle	ANG II	Vehicle	ANG II
Water intake, ml/24 h	5.20 ± 0.12	5.40 ± 0.94	5.50 ± 0.42	8.80 ± 0.60*
Urine output, ml/24 h	1.44 ± 0.26	2.46 ± 0.74	1.44 ± 0.37	4.30 ± 0.37*
Plasma creatinine, mg/dl	0.078 ± 0.002	0.077 ± 0.005	0.072 ± 0.002	0.075 ± 0.010
Urinary osmolality, mosM/kg	2720 ± 115	2140 ± 262	2630 ± 79	1281 ± 205*
Urinary Na+ excretion, mmol/24 h	0.045 ± 0.018	0.045 ± 0.021	0.031 ± 0.016	0.099 ± 0.010*
Proteinuria, mg/24 h	2.49 ± 0.47	2.64 ± 0.50	2.60 ± 0.30	6.96 ± 0.55*
Albuminuria, mg/24 h	0.23 ± 0.13	0.30 ± 0.10	0.13 ± 0.03	1.63 ± 0.33*
Body weight, g	20.1 ± 0.3	20.6 ± 1.2	21.0 ± 0.6	22.3 ± 0.7
Food intake, g/24 h	5.03 ± 0.21	4.62 ± 0.84	5.83 ± 0.18	5.31 ± 1.13

Data are expressed as means ± SE; n = 5 for all experiments. Cytochrome P-450 (Cyp)1b1^+/+ and Cyp1b1^−/− mice were infused with vehicle or ANG II for 2 wk and placed in metabolic cages for 24 h before the experiments were completed. The parameters listed above were determined as described in materials and methods.

^*P < 0.05, vehicle versus the corresponding value from ANG II-infused animals.