TABLE 2.
Mediator complex P sites
| UniProt ID | Gene ID | P site(s)a as detected by: |
||
|---|---|---|---|---|
| Large-scale phosphoproteomics | In vitro Cdk8 kinase assay (ATP dependent) | In vitro Cdk8 kinase assay (ATP independent) | ||
| Q5AHH0 | Med1 | S445, S446 | S445, S446 | |
| Q59Q94 | Med3 | S117, T125, T133 | T140 | S117, S181, S183, T119, T121, T125, T132, T133 |
| Q59U73 | Med4 | S9, S13, S17, S33, S322, T36, T37, Y321 | S9, S33, T36, T37 | S9, S13 |
| Q59KC0 | Med5 | S252 | S252 | |
| Q5A2Z1 | Med6 | |||
| Q5AEN6 | Med7 | S285 | ||
| Q59TD3 | Med8 | T37 | ||
| Q5A0D0 | Med9 | S102 | T109 | |
| Q5A3K2 | Med10 | S170 | ||
| Q59S43 | Med11 | S132, S142, T140, T144, T149 | S120, S132, S142, T140 | |
| Q59MN9 | Med12 | S594, S1033, S1579, S1680, S1682, S1713, T1039 | S63 | |
| Q5AG31 | Med14 | S385, T395 | S14, S385, S678, S763, S1170 | |
| Q5A757 | Med15 | S898, S939, S948, S1035, S1043, S1046, S1084, S1097, S1110, T903, T906, T913, T914, T937, T943, T1042 | S898, S900, S1082, S1084, T906, T913, T1030 | S898, S900, S948, S1043, S1082, S1083, S1084, T977, T1030, T1042 |
| Q59PP6 | Med16 | S380 | ||
| Q5AHZ7 | Med17 | S43, S73, T70 | ||
| Q59X40 | Med18 | S207, T162 | ||
| Med19 | ||||
| C4YHG8 | Med20 | |||
| Q5ACU4 | Med21 | S79, S83 | S79 | S79, S85, S87 |
| Q5A917 | Med22 | |||
| Q59P87 | Med31 | |||
| Q5ALX5 | Ssn2 (Med13) | S399, S478, S493, S605, S615, S650, S652, S840, T476, T494, T774, T1449 | S493, T476, T774, T1449 | S348, S605, S833, S1343, S1345 |
| Q5AHK2 | Ssn3 (Cdk8) | S209 | S209 | |
| Q5A4H9 | Ssn8 | |||
| Tlo | S39 | T38 | ||
a
P sites detected in the large-scale phosphoproteomics data set and the ATP-dependent in vitro Cdk8 kinase assay are in bold, and P sites detected in the large-scale phosphoproteomics data set and the ATP-independent in vitro Cdk8 kinase assay are in italics.