FIG 3.

TbPI-PLC1 activity as a function of Ca²⁺ concentration (A), pH (B), or substrate concentration (C and D). Experimental conditions were as described in Materials and Methods, using the same buffer with different Ca²⁺ concentrations and 5 μM PIP₂ (blue line) or PI (red line) (A) or with 10 μM Ca²⁺ and different pHs (B) or different concentrations of PIP₂ (C) or PI (D). Values are means ± standard errors of the means (SEM) (n = 3).