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Isolation of second-site suppressors of the AbA resistance of a pdr5Δ yor1 strain. (A) The indicated EMS-generated mutant strains generated in the pdr5Δ yor1 background were tested for resistance to AbA and PHS as before. (B) The strains in panel A were grown to mid-log phase and total lipids extracted as described previously (74). Levels of the two sphingoid bases in S. cerevisiae (DHS and PHS) were determined by mass spectrometry.
