FIG 5.

Interaction of Lem3-dependent flippase with ABC transporters determines resistance to sphingolipid biosynthesis. (A) The indicated strains were tested for resistance to either myriocin (Myr) or AbA. The LEM3 alleles represent lem3Δ::kanMX4 deletions that were recovered from the collection of genomic disruption mutations (75). (B) Loss of Fpk1/2 kinases phenocopies lem3Δ. Disruption alleles of FPK1, FPK2, or both were introduced into wild-type and pdr5Δ yor1strains. These mutants were tested for resistance to Myr and AbA. (C) Separation of resistance phenotypes influenced by Pdr5.