Fig. 9.

Downregulation of Aip1 attenuates the formation of intestinal epithelial cysts. Caco-2 human colonic epithelial cells were transfected with either control or Aip1-specific siRNAs. A: immunoblotting analysis shows the efficiency of Aip1 knockdown on day 4 posttransfection. B and C: cells grown for 3 days in Matrigel were fixed and labeled for F-actin and E-cadherin. Arrows show the F-actin lining of well-polarized single-lumen cysts formed by control cells. Arrowheads highlight abnormal multilumen cysts formed by Aip1-depleted cells. Data are presented as means ± SE (n = 3); *P < 0.05. Scale bar = 20 μm.