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. 2015 Mar 5;4(5):378–391. doi: 10.1016/j.molmet.2015.02.007

Figure 1.

Figure 1

Quantification of adipocyte-specific deletion of Sirt1 in mice (ATKO). A: DNA genotyping. Upper panel, Sirt1flx/flx (floxed-ATKO) and Sirt1+/+ (WT) DNA expression. Lower panel, Cre-Recombinase (all mice are Sirt1flx/flx). C+, Cre positive control; C- Cre negative control. B: Semi-qPCR showing the Sirt1 floxed exon 4 (Δ4 ATKO) in several adipose depots and other different tissues from WT and ATKO mice. 36b4 (Rplp0) expression was used as housekeeping gene. C: Sirt1 mRNA expression in intraperitoneal macrophages and epididymal white adipose tissue (eWAT). D: Semi-qPCR showing Sirt1 mRNA expression in adipocytes or Stromal vascular fraction (SVF) from WT and ATKO eWAT. E: Protein expression of SIRT1 determined by Western Blot in adipocytes isolated from eWAT. F: Acetylation levels of SIRT1 target protein p65 (NF-κB) in adipocytes from ATKO or WT mice. Hsp90 expression was used as loading control. The Western blots shown are representative of three independent experiments (**P ≤ 0.01 vs. WT). G: Relative mRNA expression of Sirt2 and Sirt3 in different adipose tissues from WT and ATKO mice after 15 weeks of HFD.