Skip to main content
. 2015 Mar 5;4(5):378–391. doi: 10.1016/j.molmet.2015.02.007

Figure 5.

Figure 5

ATKO mice show reduced inflammation and infiltration in eWAT after prolonged HFD. A–B: Plasma protein and mRNA levels of inflammatory cytokines and chemokines measured by Milliplex during short term (S) or chronic HFD (C) (n = 8 per group). C: mRNA levels of brown fat-selective genes (Ucp1, PGC-1α, Cox7a1 and Cidea), anlayzed by qPCR. D-E: Immunofluorescence of eWAT from short term or chronic HFD-fed mice. Adipose tissue was stained with Caveolin as adipocyte membrane marker and F4/80 as macrophage cell marker. Scale bar is 100 μm (n = 4/5 per group). F: Gene expression of adipose tissue infiltration markers during HFD, analyzed by qPCR. Values are expressed as means ± SEM. (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. WT at the same point, WT; n = 4/5 per group in qPCR and n = 10/12 per group for plasmatic cytokines).