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. 2015 Mar 5;4(5):378–391. doi: 10.1016/j.molmet.2015.02.007

Figure 6.

Figure 6

ATKO mice show higher hyperplasia during chronic HFD. A: Immunofluorescence of eWAT from short term HFD feeding. Adipose tissue was stained with Caveolin (blue) as adipocyte membrane marker. Scale bar is 200 μm. The images below are 4x magnification corresponding to the white box on the upper images. B-C: Quantification, area and adipocyte size frequency measurement from eWAT inmunofluorescence images. D-F: Same analysis in WT and ATKO eWAT from chronic HFD feeding mice. Values are expressed as means ± SEM. (n = 4 mice/group and 2 images/mouse were analyzed). G: Hematoxylin & Eosin of eWAT, sqWAT, and BAT from WT and ATKO mice from chronic HFD-fed mice. Fat tissues were fixed with 4% PFA, embedded in paraffin blocks and sectioned in the UCSD Mouse Phenotype Service Core (Moores Cancer Center, UCSD). Bright field photographs were taken using a Zeiss Observer.Z1 microscope. Scale bar is 100 μm. (n = 4 mice/group and 2 images/mouse were analyzed).