FIG 2.

Impact of inoculation on GS fermentation. (A) General scheme for inoculation: 1, direct application of spores; 2, spore germination in YP medium; 3, control of the colony morphology; 4, preparation of the preculture; 5, inoculation of the fermentation medium with colony cells; 6, inoculation of the fermentation medium with the preculture. (B) Influence of inoculation on the growth profile (solid circles, left axis) and GS production (empty circles, right axis). Black, inoculation with the preculture (steps 2, 3, 4, and 6); red, inoculation with colony cells (steps 2, 3, and 5).