FIG 5.

Optimization of phenylalanine concentration and impact of ornithine. Growth profile (A) and GS production (B) in GAT medium, supplemented with different concentrations of phenylalanine: 0% (filled circles, solid line), 0.025% (empty circles, solid line), 0.05% (filled squares, dotted line), 0.1% (empty squares, dotted line). (C) Phenylalanine consumption in the fermentation medium as monitored by HPLC. (D) Growth profile (solid signs, left axis) and GS production (empty signs, right axis) in GATF2 medium only (squares) and in GATF2 medium supplemented with 0.4% ornithine (circles).