FIG 6.

Physicochemical characterization of the ¹³C/¹⁵N-GS produced here. (A) MALDI-TOF spectra of commercial unlabeled GS (upper [gray], theoretical m/z = 1141.7) in comparison with the produced ¹³C/¹⁵N-GS (lower [black], theoretical m/z = 1213.7), showing 100% labeling efficiency. Experimental m/z values are shown. (B) Typical RP-HPLC trace recorded at 220 nm (GS peak is indicated). (C, D) Solid-state MAS-NMR spectra of ¹³C/¹⁵N-GS reconstituted into bilayers of 1,2-di-O-tetradecyl-sn-glycero-3-phosphocholine at a peptide/lipid ratio of 1/20, measured at 25°C: ¹⁵N-NMR spectrum (C); ¹³C-NMR spectrum (D).