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Expression of Thc_Cut1_hfb4 in E. coli BL21-Gold(DE3) at 25°C. Lane 1, uninduced cells; lanes 2 to 4, soluble cell fractions after 5 h, 10 h, and 21 h of induction; lanes 5 and 6, insoluble cell fractions after 10 h and 21 h of induction; lane 7, standard (PageRuler prestained protein ladder). Molecular masses are given on the right.
