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Kinetic parameters of cutinase from T. cellulosilytica fused to hydrophobins from Trichoderma on soluble substrates
| Cutinase | PNPA |
PNPB |
||||
|---|---|---|---|---|---|---|
| Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) | |
| Thc_Cut1 | 1.4 ± 0.1 | 363 | 291 | 0.8 ± 0.1 | 325 | 406 |
| Thc_Cut1_hfb4 | 2.4 ± 0.2 | 99 | 42 | 1.3 ± 0.1 | 86 | 68 |
| Thc_Cut1_hfb7 | 2.3 ± 0.2 | 129 | 55 | 0.9 ± 0.1 | 112 | 130 |
| Thc_Cut1_hfb9b | 1.5 ± 0.1 | 105 | 71 | 0.8 ± 0.1 | 49 | 62 |
