FIG 6.
Mutational analysis of the DptR3-binding site. (A) DNA probes containing an intact palindromic sequence in the DptR3-binding site or mutated sequences. Probes 1a and b, 50-bp WT DNA containing intact palindromic sequences a and b, respectively. Mutations were introduced into probe b to produce mutated probes bm1, bm2, and bm3, respectively. Altered nucleotides are underlined. (B) EMSAs with various DNA probes. Each probe was incubated with 50, 100, 150, and 200 ng of His6-DptR3 (concentrations increase from left to right in each series of four lanes, as indicated by the triangles above the blots).