TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| E. coli JM109 | Plasmid storage and expression of lcp | |
| E. coli XL1-blue | QuikChange transformation strain | Stratagene |
| Xanthomonas sp. strain 35Y ΔroxA-attB | No clear zone formation on latex agar | 12 |
| Xanthomonas sp. strain 35Y ΔroxA-attB pNH1-roxA-attP in chromosome (SN4230) | Expression of RoxA from rhamnose promoter in Xanthomonas sp. strain 35Y-CM, Kmr, Cmr; clearing zone formation in the presence of rhamnose plus latex | 12 |
| pMK::lcp (SN5314) | GeneArt vector, source of lcp, Kmr | 17 |
| pUC9::lcp (SN5339) | Cloning vector for lcp, Ampr | This study |
| p4782.1 (SN3513) | Mobilizable broad-host-range expression vector, Kmr | 21 |
| p4782.1::strep-lcp (SN5496) | Coding sequence of strep-lcp under rhamnose promoter control, Kmr | This study |
a
Kmr, kanamycin resistance; Ampr, ampicillin resistance; Cmr, chloramphenicol resistance.