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. 2015 May 5;81(11):3782–3792. doi: 10.1128/AEM.00239-15

TABLE 1.

Primer sequences of the indicated genes used for quantitative RT-PCR

Organism Gene Oligonucleotide sequence (5′–3′)
Source or reference
Forward Reverse
E. coli gapAa AAGTTGGTGTTGACGTTGTCGCTG ATAACCACTTTCTTCGCACCAGCGG 34
E. coli fimA ACTCTGGCAATCGTTGTTCTGTCG ATCAACAGAGCCTGCATCAACTGC 28
E. coli papA2 ACGGGTGAAATTTGATGGAGCCAC AATTCGCAACTGCTGAGAAGGCAC 36
E. coli flhD TCCGCTATGTTTCGTCTCGGCATA ACCAGTTGATTGGTTTCTGCCAGC 36
E. coli fliC ACAGCCTCTCGCTGATCACTCAAA GCGCTGTTAATACGCAAGCCAGAA 34
E. coli acrB CTGATCATCGTGGTCGGCATGGC CCAGTCCTTCAAGGAAACGAACGC 31
E. coli motB GCGTTACGTCCACATCTCAA ATGTCGCGCATATAGGGTTC This study
E. coli uvrY GCCAGTTTGTCTGAACGTGA CTGTTCACCGTTTTCGGACT This study
E. coli marC GTCGGAAGAGCTGGAAGATG GAACTCTGACGCACTGTGGA This study
E. coli emrA ACAGGTAGCGCGTTCTCACT AGCGTGGATAAACCGATACG This study
E. coli chuA AGCAAACAACCTGGCTATGG CTCTTTATCGAAGGCGTTGC This study
E. coli fimH GGAACCATTCAGGCAGTGAT CGGTTTTACAGGCGAATGAC This study
P. mirabilis rpoAa GCAAATCTGGCATTGGCCCTGTTA TAGGGCGCTCATCTTCTTCCGAAT 35
P. mirabilis flhD AAGGCTTCCGCAATGTTTAGAC GTTGCAAATCATCCACTCTGGA 29
P. mirabilis flaA TGCTGGTGCAACTTCATACG TTTGTCAGCACCTTCCAGTG This study
P. mirabilis ureA GGGGTGCCAGAGATGATAAA CCGGGGATCATGTTATTACC This study
P. mirabilis ureD CCTTACGCACATGCCCTATT CTTGTGCAACCGTCAATGTC This study
P. mirabilis atfB ATTTAGCTGCAGCCGACAGT GAGTCTGTGCGCCATAATCA This study
P. mirabilis marC ATCTCGGCCACAGTGGTATC AATAAAGCGGGGAGATCAGC This study
P. mirabilis acrA GCTGAAATTGCTCGCCTAAC GCAACAGCTTGAGCGTACTG This study
P. mirabilis cysJ CAATGCACGTCGTTTAGCTG TTCCCCCTGTGTTGAGGTAA This study
P. aeruginosa rpoDa GCCGAGATCAAGGAAATCAA GTGTACTTCTTGGCGATGGAA 33
P. aeruginosa lasB AAGCCATCACCGAAGTCAAG CGGATCACCAGTTCCACTTT 33
P. aeruginosa plcH TGACTTCGCTGTTCGACTTC TGGGCTCGTAGGACCAGTAT 33
P. aeruginosa phzS CGTCGGCATCAATATCCAG ATCGAGTACTGCGGATAGGC 32
P. aeruginosa pvdA GTTCCACCACAGCCAGTACC CTGTCGTTGAGGTCGATGAA 32
P. aeruginosa fliC AACTTCGACGTAACCGTTGG TGGTCAGTACACCCTTGTCG 32
P. aeruginosa mexA CGACCAGGCCGTGAGCAAGCAGC GGAGACCTTCGCCGCGTTGTCGC 30
P. aeruginosa mexX TGAAGGCGGCCCTGGACATCAGC GATCTGCTCGACGCGGGTCAGCG 30
P. aeruginosa oprM TCAACCTGCGCTACACCA GCTACCGTCCTCCAGCTTC This study
P. aeruginosa cupA1 GCGGCAAACACTATCACATTC AACAGGGTGGTGAAATGCTC This study
P. aeruginosa fleQ GATCAGCTGACCTGCAACAG GCAGGTACTCGTCCCAACTG This study
a

Housekeeping genes (endogenous control).