TABLE 2.

Plasmids and DNA fragments used in this study

Plasmid or DNA fragment	Description	Source or reference
Plasmids
Geneart-chloride	Kanr; source of the CIP system	Geneart
pNZ8048	Cmr; inducible expression vector carrying the nisA promoter	14
pNZC	pNZ8048 derivative containing the CIP	This work
pNZ8048L	pNZ8048 derivative containing lacZ under the nisin-inducible promoter	This work
pNZCL	pNZC derivative containing lacZ under the CIP	This work
pBac	Spcr; source of Bac fragment	6
pNZCA3	pNZC derivative containing Bac under the CIP	This work
pBK1	Cmr; source of lacZ	6
DNA fragments
CIP systema	1,317-bp fragment containing the chloride-inducible promoter (Pgad) and the gene encoding the activator protein (gadR) under the control of a constitutive promoter (PgadR)	15
Bac	1,610-bp fragment containing the enterocin A structural gene (entA) with its immunity gene (entiA), the enterocin P structural gene (entP) with its immunity gene (entiP), and the hiracin JM79 structural gene (hirJM79) with its immunity gene (hiriJM79)	6

^aCIP, chloride-inducible promoter.