TABLE 2.
Plasmid or DNA fragment | Description | Source or reference |
---|---|---|
Plasmids | ||
Geneart-chloride | Kanr; source of the CIP system | Geneart |
pNZ8048 | Cmr; inducible expression vector carrying the nisA promoter | 14 |
pNZC | pNZ8048 derivative containing the CIP | This work |
pNZ8048L | pNZ8048 derivative containing lacZ under the nisin-inducible promoter | This work |
pNZCL | pNZC derivative containing lacZ under the CIP | This work |
pBac | Spcr; source of Bac fragment | 6 |
pNZCA3 | pNZC derivative containing Bac under the CIP | This work |
pBK1 | Cmr; source of lacZ | 6 |
DNA fragments | ||
CIP systema | 1,317-bp fragment containing the chloride-inducible promoter (Pgad) and the gene encoding the activator protein (gadR) under the control of a constitutive promoter (PgadR) | 15 |
Bac | 1,610-bp fragment containing the enterocin A structural gene (entA) with its immunity gene (entiA), the enterocin P structural gene (entP) with its immunity gene (entiP), and the hiracin JM79 structural gene (hirJM79) with its immunity gene (hiriJM79) | 6 |
CIP, chloride-inducible promoter.