Skip to main content
. 2015 May 5;81(11):3745–3752. doi: 10.1128/AEM.00127-15

FIG 1.

FIG 1

Expression of the E2-Tα1 fusion protein from L. plantarum/pYG-E2-Tα1 was identified by SDS-PAGE and Western blotting. M, molecular mass marker. An ∼46-kDa protein was expressed in L. plantarum/pYG-E2-Tα1 cells induced by xylose (lanes 1 and 2) but not in noninduced cells (lanes 3 and 4). Western blotting showed that an immunoreactive band was developed in a position similar to that observed by SDS-PAGE using mouse anti-CSFV E2 antibody (lane a) and mouse anti-Tα1 antibody (lane e), respectively. However, there was no corresponding band observed in noninduced L. plantarum/pYG-E2-Tα1 (lanes b and f). The E2 protein expressed by L. plantarum/pYG-E2 was identified via SDS-PAGE (lane 5) and Western blotting (lane c), and there was no corresponding band observed in the noninduced cells (lanes 6 and d).