TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Characteristicsa	Source or reference
Strains
L. rhamnosus GG	Wild type	ATCC 53103
L. rhamnosus BL394	LGG with an 963-bp in-frame deletion in fucP	This study
L. rhamnosus BL395	LGG with a frameshift at the fucI EcoRI site	This study
L. rhamnosus BL396	LGG with an insertion of a pRV300-derivative at the fucR gene; Eryr	This study
L. casei ATCC 393	Wild type	ATCC 393
L. zeae KCTC3804	Wild type; very similar to L. casei isolates	ATCC 15820
E. coli DH10B	F− endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 ϕ80lacZΔM15 araD139 Δ(ara leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ−	Invitrogen
Plasmids
pRV300	Nonreplicative plasmid for Lactobacillus; Ampr Eryr	20
pRΔFucP	pRV300 with a 1,221-bp fragment containing fused 5′ and 3′ fucP fragments	This study
pRFucI	pRV300 with a 997-bp fucI fragment containing a frameshift at the EcoRI site	This study
pRFucR	pRV300 with a 437-bp fucR internal fragment cloned at EcoRV site	This study

^aEry^r, erythromycin resistance; Amp^r, ampicillin resistance.