FIG 2.

Screening of activity of CbXyn10C on various polysaccharides. The reaction mixture contained 10 μM CbXyn10C in pH 6.5 McIlvaine buffer with each of a series of polysaccharides, including 10 mg/ml of beech wood xylan, WAX, barley β-glucan, lichenin, laminarin, CMC, Avicel, filter paper, gum arabic, debranched arabinan, sugar beet arabinan, and xyloglucan; 5 mg/ml of konjac glucomannan, carob bean gum, locust bean gum (LBG), and guar gum; and 2.5 mg/ml of PASC and pectin. The release of reducing sugars in the reaction mixture was measured by using the DNS method.