FIG 3.

Effect of removing the amorphous region from Avicel on the activity of CbXyn10C. Avicel (10 mg/ml) was first incubated with 10 μM CelDTM1 in pH 6.5 McIlvaine buffer at 70°C for 16 h. As a control, Avicel was incubated with CelDTM1 that had been inactivated by boiling at 100°C for 10 min. Then, the samples were boiled at 100°C for 10 min to degrade the CelDTM1. Ten micromolar CbXyn10C was added to the pretreated Avicel, and the reaction was carried out at 75°C. At different time points (30 min, 60 min, 90 min, 120 min, and 150 min), samples were taken out and the reducing sugars were measured using the DNS method.