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Hydrolysis products of WAX (A), Avicel (B), xylooligosaccharides (C), and cellooligosaccharides (D) by CbXyn10C analyzed by thin-layer chromatography (A, C, and D) or HPAEC-PAD (B). One micromolar CbXyn10C was incubated with 20 mg/ml of Avicel, 2.5 mg/ml each xylo-/cellooligosaccharides, or 1 mg/ml of WAX in McIlvaine buffer at pH 6.5 and 75°C for 16 h. X1, xylose; X2 to X6, xylobiose to xylohexaose; G1, glucose; G2 to G6, cellobiose to cellohexaose. Lanes M, molecular mass markers.
