TABLE 1.
Primers used in this study
| Primer | Sequencea (5′–3′) | Usage |
|---|---|---|
| CbXyn10C-F | GACGACGACAAGATGCCTGACTGGAACATTCCAAGTTTATATG | Cloning of C. bescii xyn10C into pET46-Ek/LIC |
| CbXyn10C-R | GAGGAGAAGCCCGGTTATGATGTCCCTGATGCTTCTATTACTGC | |
| CbXyn10C-CBM3b-F | GGGAATTCCCTGACTGGAACATTCCAAGTTTATATG | Cloning of C. bescii xyn10C-CBM3b into pET-28a(+) |
| CbXyn10C-CBM3b-R | GGCTCGAGGGTGTCGGTGTCACTGTCGGTG | |
| CbXyn10C-2CBM3b-F | GGGAATTCCCTGACTGGAACATTCCAAGTTTATATG | Cloning of C. bescii xyn10C-CBM3b-CBM3b into pET-28a(+) |
| CbXyn10C-2CBM3b-R | GGCTCGAGGGTTGGAGTTGGTGCTGGTGTG | Cloning of celDTM1 into pET28a |
| CelDTM1-F | GGGAATTCCAGAGCATACTGTATGAAAAGG | |
| CelDTM1-R | GGCTCGAGTTACTCAAAAAGGATATTGGTAAATC | |
| Q94A-F | CTTTAGTGTGGCACAGCGCGACGCCTGATTGG | Mutation of Q94 to alanine |
| Q94A-R | CCAATCAGGCGTCGCGCTGTGCCACACTAAAG | |
| L306A-F | TTATTCATGGGCAAGATCTTTTAACG | Mutation of L306 to alanine |
| L306A-R | CGTTAAAAGATCTTGCCCATGAATAA | |
| E140A-F | ACGTTGTAAATGCAGCCATTGATGAGAATCAGTC | Mutation of E140 to alanine |
| E140A-R | TCATCAATGGCTGCATTTACAACGTCCCATGC | |
| E248A-F | ATAACAGCGCTTGATATGAGTTTATACAATTACGG | Mutation of E248 to alanine |
| E248A-R | CTCATATCAAGCGCTGTTATATGAATTTCTATGC |
a
The underlined nucleotides are the EcoRI (GAATTC) and XhoI (CTCGAG) restriction sites.