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Hydrolysis of mixed substrates by CbXyn10C
| Reaction no. | Substrate concn in reaction mixture (mg/ml) |
Observed value (ΔAa/min/mg) | Total calculated velocity of hydrolysis (ΔA/min/mg) with: |
||
|---|---|---|---|---|---|
| Beech wood xylan | Barley β-glucan | Same active center | Different active center | ||
| 1 | 16 | 4 | 0.20 ± 0.00 | 0.18 | 0.27 |
| 2 | 12 | 8 | 0.21 ± 0.00 | 0.18 | 0.29 |
| 3 | 10 | 10 | 0.21 ± 0.00 | 0.18 | 0.29 |
| 4 | 8 | 12 | 0.22 ± 0.00 | 0.17 | 0.29 |
| 5 | 4 | 16 | 0.21 ± 0.00 | 0.16 | 0.29 |
ΔA, change in absorbance at 540 nm.
