Table 1.
Numbers of Ta-1 Inserts Collected and Fractions of Polymorphic Inserts
Cloning
|
||||||||
---|---|---|---|---|---|---|---|---|
Druze | Biaka | Chinese | Melanesian | Total | Databasea | Totalb | ||
Clones collected | 1344 | 1576 | 1851 | 1980 | 6751 | — | — | |
Clones sequenced | 613 | 156 | 253 | 234 | 1256 | — | — | |
Number of different Ta-1 inserts | 167 | 58 | 35 | 17 | 277 | 67 | 344 | |
Flanking sequences located in database | 142 | 52 | 26 | 12 | 232 | 67 | 299 | |
Tested by PCR | 116 | 28 | 21 | 9 | 174 | 43 | 217 | |
% Polymorphic | 52% | 75% | 71% | 100% | 60% | 72% | 62% | |
Insertion sites occupied in database | 102 | 19 | 13 | 4 | 138 | 67 | 205 | |
Tested by PCR | 86 | 14 | 12 | 2 | 114 | 43 | 157 | |
% Polymorphic | 38% | 57% | 50% | 100% | 43% | 72% | 51% | |
Insertion sites empty in database | 40 | 33 | 13 | 8 | 94 | — | 94 | |
Tested by PCR | 29 | 14 | 9 | 7 | 59 | — | 59 | |
% Polymorphic | 90% | 93% | 100% | 100% | 93% | — | 93% | |
Flanking sequences not located in database | 25 | 6 | 9 | 5 | 45 | — | 45 | |
Flanks are repeated DNA | 17 | |||||||
Flanks not present in db | 28 | |||||||
Tested by PCRc | 16 | 2 | 4 | 0 | 22 | — | 22 | |
% Polymorphic | 50% | 0% | 25% | — | 41% | — | 41% |
Elements from the database that were missed by cloning but retrieved by a BLAST search.
Total number of Ta-1 inserts including cloning and database search.
The polymorphism of these elements was tested with a single PCR using only the flanking primer cognate to the 3′ flanking sequence of the inserts.