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Optimized PCR Conditions
| Primer 1 | Primer 2 | Annealing temp | Cycle number |
|---|---|---|---|
| Ty1: JB2930 | tGly: JB2821 | 60 | 30 |
| Ty1: JB2930 | tThr: JB3084 | 54 | 30 |
| Ty1: JB2930 | tG(GCC)D1: JB6071 | 61 | 30 |
| Ty1: JB3361 | tGly: JB2821 | 60 | 30 |
| Ty1: JB3361 | tThr: JB3084 | 60 | 32 |
| Actin: JB4307 | Actin: JB4312 | 54 | 20 |
