Table 2.
Protein Interactions Detected by Y2H Library Screens
| Bait construct | Library screen interactionsd |
|---|---|
| Rrp41 | PM/ScI-75 (5), FLJ20297 (3), C1or19 (3), DKFZp566J2046 (3), FLJ33418, EEF1A1, NEK1, BC006102, BM770606, AKR1A1, GTF2IRD1, LRRC5, FLJ21047, PLINP |
| Rrp42/KIAA0116 | — |
| Rrp46c | OIP2 (94), Lsm5 (8), Rrp40 (4), EBP1 (3), NUP210 (2), CsI4, PKM2, SFPQ, FLJ30932, DCK5RAP1, POLR2L |
| PM/ScI-75/Rrp45 | — |
| Mtr3 | EIF3S2 (5), Rrp42 (5), GRIM19 (2), SF2p32, SFRS3, CPSF5, RAB3-GAP150, PSMB1, ERRalpha, AD023, MOCS3, FLJ22678, WBSCR18, KIAA0182 |
| Oip2 | FHOD1 (11), RASSF1 (9), MKRN1 (8), KIAA1604, AI685517, FOXN3 |
| Rrp4c | Rrp42 (49), Znf408 (6), RNF8 (2), FLJ40491 |
| Rrp40 | Rrp46, CDK5RAP1 |
| CsI4 | NUP160 (17), Rrp41 (3), AV753896 |
| PM/ScI-100/Rrp6 | CIB1 (3), C14orf92, SCRIB, PTGES2, BM451300, |
| exonuclease [1-456] | PM5 (2), GA17, FACT-80, USP16, EPPB9, H17, FLJ22678, FLJ23784, AL110135, AL512687 |
| HRDC [503-583] | FLJ11934 (2), TARDBP, RPE, URP2, |
| [584-860] | RUVBL2, AJ431196, AI620703 |
| Kiaa0052b | — |
| Mpp6c | EIF3S4 (9), FTL (4), SNX9 (4), THOP1, FLJ14502, DNLC2A, MT2P1, BM806939, RPS20, BRG1, DYN2, AATF, MacGAP |
| Rrp44 | — |
| PinC [1-182] | AD-003 (2), ZNF151 |
| PinC + CSP [1-387] | — |
| RNase II + RNB [388-958] | — |
| Ski2b | — |
| helicaseb [1-708] | — |
| [709-1246] | SmB (5), AL517440 (2), FLJ34136, GALNT13, BF248318, U80752, WDR1, CDC2L2 |
| Lsm1 | VPS11 (20), Lsm2 (12), NARS (9), PSMB8 (7), Lsm3 (5), PSMB5 (2), FLJ20254 (2), UXT, N4BP1, GRHPR, HSPC1, DKFZP564J0123, AF090094, AW27564, AW974116, AW975586, KIAA0179 |
| Lsm2e | Lsm8 (88), Lsm3 (75), SmE (5), Znf408 (3), DNAJL2 (2), Lsm7, SmD2 |
| Lsm3 | Lsm2 (51), Lsm8 (36), Lsm10 (21), SmD3 (14), SmE (8), Lsm7 (8), XPC, BC009518 |
| Lsm4 | Lsm8 (32), Lsm7 (27), FLJ13289 (18), SmE (11), Nmi (4), PEPP-2 |
| Lsm5 | Lsm7 (75), SmF (13), SmE, Lsm3 |
| Lsm6 | Lsm5 (40), SmD2 (37), Lsm7 (32), Lsm3 (7), MCRS1 (4), POLD2 |
| Lsm7 | Lsm5 (61), SmD3 (3), Lsm4 (3), RAB7L1 (2), NPY1R, MYH13 |
| Lsm8c | Lsm2 (17), NY-REN-45 (8), FLJ23534 (8), LSM4 (5), TALDO1 (4), SmD1 (3), PDCD2 (3), DKFZp565A176 (3), PGD (2), ASK (2), FLJ23469 (2), RPL24, SMBP2, PNUTL1, MTHFR, DKFZP434F091, MGC10966, GTF21, ITSN2, FLJ11280, DKFZp434K0621, IFNLR1 |
| Dcp2 | POLA2 (7), NAGK, PSMB1 |
| Dcp28 (Δ exons 8 and 9) | RCD-8 (7) |
| Dcp1Ab | — |
| Dcp1Bb | — |
| DcpS | — |
| Xrn1/Sep1 | BAT3 (2), LDHA (2), PABPC4, BC001200, ALDOA, MGC13198, AP1G2, STAM2A, CHD4, |
| Exonuclease [1-300] | SMARCD2, CCT5, GRIM19, FLJ10359, FLJ21347, AI821928 |
| [578-1470] | — |
| RBD [1440-1707] | hnRNPA1 (2), KIAA0261 (2), DPP2 (2) and many others |
| Xrn2/Rat1 | PSMA3 (6), EIF3S6IP, AJ420488, AW977134 |
| Exonuclease [1-280] | EIF6 (2), EIF5A, DKFZP434J214, COMT, MOCS3, TOLLIP, TARDBP |
| Dom3d | AI580336 |
| PARN | DKFZp434N0650 (12) |
| TTPb | — |
| Auf1/hnRNPDc (variant 3) | SF2p32 (30), Rrp41, Lsm5 plus many others |
| HuR | — |
| Upf1 [1-415] | — |
| Helicase [416-701] | — |
| [702-1119] | HIRA (2), BC040175, N21259, PLEKHB2, FLJ10631, ACAS2, BAT5, PEPP2, KIAA0460, GNPTAG |
| Upf2 [1-167] | — |
| MIF4G1 + 2 [166-758] | — |
| [757-1272]c | RPS15 (12), RPL5 (5), REA (3), MAP1A (3), RPS25 (2), ART4 (2), UPF3B + many others |
| Upf3A (variant2) | GSK3B (2), BM310104 (2), IFITM2 |
| Upf3B | MCRS1, FLJ20343 |
| Znf408 | RGS19IP1 (6), RIL (2), NOH61 (2), Lsm2, Znf408, NOA36, DKFZP434G156, BG403593, BQ069192, MIF, TOP3B, CAF1B |
aFor descriptions of baits, see Table 1.
Previously published interactions are in bold, and interactions detected between the orthologous yeast or Drosophila proteins in genome-wide data sets are underlined.
Self-active bait.
Weakly self-active bait (— His selection).
The number of colonies isolated for each bait is indicated in parentheses when greater than 1. Singly isolated preys are not listed for baits that isolated >20 different preys. A few interactions with extracellular proteins or those known to localize to the secretory pathway or mitochondria are not listed, because they are unlikely to represent physiological interactions. A full list of interacting preys, accessions, and descriptions are available from http://www.hgmp.mrc.ac.uk/Research/RNA and as Supplemental material.
Lsm2 and Dom3 library screen interactions were previously reported (Lehner et al. 2004).