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. 2004 Jul;14(7):1413–1423. doi: 10.1101/gr.2111304

Figure 4.

Figure 4

Experimental validation of MGC5601 gene alternative splicing isoforms. (A) Gene structure for exons XVI-XIX (boxes) of the MGC5601 gene located on chromosome 12. Introns are represented by lines. Two alternative exons are shown on TFU reads, and a hypothetical combination of these two exons is also shown. Sequence F07R has an extra exon between exon XVII and XVIII. Sequence A01R has an extended exon XIX. Four primers were designed for validation tests, as indicated in the figure (P1–P4), and each pair of primers were assayed against all 22 cDNA preparations without pooling. (B) We detected all four of these alternative splicing isoforms in MGC5601. Numbers one through four indicate the tissues from which the cDNA was obtained (1, multiform glioblastoma; 2, glioblastoma; 3, prostate carcinoma; and 4, primary kidney cell culture). The sizes of the bands obtained are indicated. L indicates 100-bp ladder.