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Knock-down of endogenous endophilin 2 has no effect on viral production. Synthetic siRNAs were transfected twice into 293T cells. A Mo-MuLV proviral DNA pNCA was cotransfected with siRNAs at the second transfection (see text). (a) Proteins in cell lysate and virion particles were analyzed by western blotting with anti-endophilin 2 and anti-capsid antibodies. (b) Virion production was monitored by assaying reverse-transcriptase activity.
