Table 2.
ChIP antibodies and amounts used in IPs
| Antibody Target | Manufacturer | Product Code | Amount in IP |
|---|---|---|---|
| H3K9me3 | Millipore | 17-625 | 4 μg |
| H4K4me3 | Active Motif | 39159 | 4 μl (whole serum) |
| Pan H4Ac | Millipore | 06-598 | 4 μg |
| Pan H3Ac | Millipore | 06-599 | 4 μg |
| H3K9Ac | Active Motif | 39137 | 10 μl (whole serum) |
| H3K14Ac | Active Motif | 39599 | 10 μl (whole serum) |
| H3K27Ac | Active Motif | 39135 | 4 μl (whole serum) |
| H3K18Ac | Active Motif | 39693 | 4 μl (whole serum) |
| p300 | Santa Cruz | Sc-9001X | 5 μg |
| P/CAF | Santa Cruz | Sc-6300 X | 5 μg |
| BRD2 | Santa Cruz | Sc-46805 | 3 μg |
| BRD3 | Santa cruz | Sc-99192 | 3 μg |
| BRD4 | Bethyl Labs | A301-985A50 | 5 μg |
| p65 | Santa Cruz | Sc-372X | 5 μg |
| CEBP | Santa Cruz | Sc-150X | 5 μg |
| RNA polymerase II | Santa Cruz | Sc-9001 | 5 μg |
IPs, immunoprecipitations; CEBP, C/EBPβ.