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. 2015 Mar 10;47(5):177–186. doi: 10.1152/physiolgenomics.00138.2014

Fig. 4.

Fig. 4.

Promoter analysis of the gene renin (Ren). A: ECR browser analysis of the intergenic space (roughly 30 kB) between Renin (REN) and the syntenic KiSS-1 metastasis-suppressor gene (KISS1) for the rat (rn4), mouse (mm10), dog (canFam2), and monkey (rh2Mac2). Regions in blue are protein coding exons, yellow are untranslated regions (UTRs), and those in red and green share conservation with human based on 20 bp stretches sharing ≥ 90% homology. B: ECR browser analysis for the proximal promoter of REN with regions in red and green showing conservation of 50 bp stretches with 50% homology. C: the large ECR in the proximal promoter of REN was analyzed for shared transcription factor (TF) binding sites between human and rat using the RVISTA software selecting for all possible TFs. D: the sequence of REN(−1444/+8) pGL3 promoter construct identifying the TATA box (red, highlighted yellow) located within the proper distance of the 5′-UTR (highlighted yellow). Additionally the conserved TF sites are colored relative to that in C. The 3 potential SOX binding sites (highlighted in red) fall outside of the ECR.