Skip to main content
. 2015 Mar 10;47(5):177–186. doi: 10.1152/physiolgenomics.00138.2014

Fig. 5.

Fig. 5.

Regulation of the REN promoter by various SOX proteins. A: activation of the REN promoter with 2 concentrations of transfected SRY (light gray) or repression by 2 concentrations of SOX3 (dark gray). In addition a double transfection of SRY and SOX3 (checkered) showed downregulation of the promoter similar to that of SOX3 transfected cells. B: transfection of CHO cells with either the control pEF1 vector (black) or 1 of the SOX pEF1 constructs (gray) along with the pGL3 REN(−1444/+8) luciferase vector. Error bars are shown as the SE. Each sample is compared with the control for each promoter. *Significant differences (P ≤ 0.05). C: the REN(−1444/+8) promoter construct was mutated to remove each of the 3 SOX binding sites; this construct was named REN(−1444/+8) SOX mut. The mutant pGL3 construct (gray) was transfected with each of the SOX pEF1 expression vectors resulting in an increase in promoter activity relative to the wt pGL3 promoter construct (black) for all but the SOX30 vector (n = 1).