Table 1.
Primers used for mutagenesis of luciferase promoters
| Construct Name | Primer Name | Primer Sequence |
|---|---|---|
| Renin SOXdel1 | R1-RenSoxdel | 5′-CAT CAG GTT TGA CTT ACA GG-3′ |
| L1-RenSoxdel | 5′-CTT GAA TGT AAT CAG ACA CAG-3′ | |
| Renin SOXdel2 | R2-RenSoxdel | 5′-TGT TAA AGA TGT ATA GGA AC-3′ |
| L2-RenSoxdel | 5′-TGT CTG TAT ATT TCC TTA TC-3′ | |
| Renin SOXdel3 | R3-RenSoxdel | 5′-TGA AAC CCC ATC TCC ACT AA-3′ |
| L3-RenSoxdel | 5′-AGG CTG GTC TGG AAC T-3′ | |
| MAS(−1123/+4) | R-MAScleavage | 5′-GGC TAG CAC GCG TAA GAG CTC G-3′ |
| L3-MAScleavage | 5′-GGA GTT CAA GAC CAG CCT CAC CAA CA-3′ | |
| MAS(−377/+4) | R-MAScleavage | 5′-GGC TAG CAC GCG TAA GAG CTC G-3′ |
| L1-MAScleavage | 5′-AGA ACT GGA AAT GGG TCC CGC ACA-3′ | |
| MutSOX-1855 | R-MASSoxdel1 | 5′-CAA CGG CCC TCA AAC TGA TGT CAG TGC GTG AA-3′ |
| L-MASSoxdel1 | 5′-CTG TTG CCG CAT TTT GCT TGA CTG CCT CCC TAC-3′ | |
| MutSOX-86 | R-MASSoxdel7 | 5′-AAA GGG AGA AAT TCA GCT ATA AAT CAT AAT CTT CAT G-3′ |
| L-MASSoxdel7 | 5′-TAT TCC AAT TCG GCG ATT TTC ATG GCT TTT TGT GTT TGT T-3′ | |
| RecSOX-86 | R-MASSoxdel7W | 5′-AAA GGG AGA AAT TCA GCT ATA AAT CAT AAT CTT CAT G-3′ |
| L-MASSoxdel7W | 5′-TAT TCC AAT TCA ACA ATT TTC ATG GCT TTT TGT GTT TGT T-3′ | |
| Mut2SOX-86 | R-MASSoxdel8 | 5′-AAA GGG AGA AAT TCA GCT ATA AAT CAT AAT CTT CAT G-3′ |
| L-MASSoxdel8 | 5′-TAT TCC AAT TCA ACT TGT TTC ATG GCT TTT TGT GTT TG-3′ |
To make each of the mutated promoter constructs the primers in this table were used. The 1st column shows the construct name as used in the study, the 2nd column the individual primer names, and the 3rd column the primer sequences. For mutations, the SOX binding site is underlined and bolded with the DNA bases to be mutated shown in italics.