Figure 2. Pitstop-2 impairs binding of Impβ to the NPCs.

Binding of Alexa-488 Impβ to the NPCs in presence of 0.1% DMSO (A), 30 μM Pitstop-2 negative control (D) and 30 μM Pitstop-2 (G) was examined with confocal microscopy. Concomitant immunostaining with mAb414 (D, E, H) was used to confirm the specificity of the Impβ binding to the NPCs (C, F, I). Scale bar = 5 μm. mAb414 staining was not affected by any of the investigated compounds and was therefore used as normalization control to quantify the extent of Impβ binding inhibition by Pitstop-2 (J). 100 individual NPCs from 20 different cells were analyzed for each condition. Statistical significance of the differences was assessed by Mann-Whitney test (nonparametric groups) at p ≤ 0.05. Quantification approach is further detailed in Fig. S5