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. 2015 May 6;5:9999. doi: 10.1038/srep09999

Figure 1.

Figure 1

Gonad reversal and small RNA profiling in swamp eel. (a) Sex reversal from female into male via intersex revealed by H & E staining of ovary, ovotestis and testis (magnification x10). T, testis; O, ovary; Odg, degenerated ovum. (b) Read percentages for small RNA sequences in ovary, ovotestis and testis respectively. By aligning to reference genome, Rfam database, GenBank noncoding database and repeat annotation files, all mapped reads are classified into 12 categories as intron, exon, rRNA, snoRNA, tRNA, snRNA, miRNA, repeat sequences, piRNA and unannoted groups (including novel miRNAs). (c) Overview of the miRNA precursors including conserved and novel miRNAs. (d) Venn charts indicate expression patterns of conserved miRNAs and novel miRNAs among three gonads. Conserved miRNAs are mainly co-expressed in three types of gonads, while most novel miRNAs are specifically in one type of gonads. (e) Graphic representation of the overlapping and non-overlapping piRNAs among three stages of the gonads. In total, 139,379, 166,903 and 249,000 piRNAs were detected in ovary, ovotestis and testis, respectively.