Table 1.

Oligonucleotides used in this study

DOI: http://dx.doi.org/10.7554/eLife.05506.023

Target	Accession	Primer name	Primer sequence
Piwi1	JG772275.1	CniwiFort7	5′-gatcataatacgactcactatagggagagttgatttcacaatcggttagac-3′
		CniwiRevSp6	5′-agtgcatttaggtgacactatagaagtgtactactactactactggttattt-3′
		PiwiIRNAiT7Fw	5′-gcgtaatacgactcactatagggagagctgtgtggaaagaccagtc-3′
		PiwiIRNAiSP6Rv	5′-tgcatttaggtgacactatagaagtgcgtcaaatccaatcaccatc-3′
		Piwi1qPCRfw	5′-aagtatggcctggcatctca-3′
		Piwi1qPCRrv	5′-cactgtctgctgtcgtaaaacc-3′
		Piwi1qPCRprobe	5′-tgcagtatgaacaagatgtgatgttgtgtgctgatgttcag-3′
Pl10	AB048849.1	Pl10ForT7	5′-gatcataatacgactcactatagggagattctggcaaaacagctgcattt-3′
		Pl10RevSP6	5′-agtgcatttaggtgacactatagaagtgagcttcatccagacaaagaaac-3′
		Pl10rnaiFWT7	5′-gcgtaatacgactcactatagggagagcgtaacacccattttg-3′
		PL10rnaiRVsp6	5′-tgcatttaggtgacactatagaagtgtaaatcacgcgca-3′
Ncol1	JX486117.1	Ncol1-T7fwd	5′-gatcataatacgactcactatagggacgtccaggaccaccaggagta-3′
		Ncol1-Sp6rev	5′-tagcaatttaggtgacactatagaactgggcaacagtattgtggacaaga-3′
Vasa	EF467228.1	HeVASAforT7	5′-taatacgactcactatagggagaaggttcaaagtggttgccattt-3′
		HeVASArevSP6	5′-atttaggtgacactatagaagagtactgccaactttaccaat-3′
		VasaRNAiFWT7	5′-gcgtaatacgactcactatagggagagttgaaatgctgggacaagaagg-3′
		VasaRNAiRVsp6	5′-tgcatttaggtgacactatagaagtggcggtagcgataagaacagtc-3′
Wnt3	AM279678.1	Wnt3ForwardPrimerT7	5′-gatcataatacgactcactataggggagtccgccttcattagtgg-3′
		Wnt3ReversePrimerSP6	5′-tagcaatttaggtgacactatagaatgggcggagtcgtatctatc-3′
cMyc	JF820068.1	cMycInSituFWt7	5′-gatcataatacgactcactatagggcctttaacgcctcccagttct-3′
H2A		H2aRNAiFwd1T7	5′-gatcataatacgactcactatagggatgtctggacgtggaaaagg-3′
		H2aRNAiRv1SP6	5′-tagcaatttaggtgacactatagaaccaatatctcagcagataaatattccaag-3′
		H2aRNAiFwd2T7	5′-gatcataatacgactcactataggggagttggctggtaacgcag-3′
		H2aRNAiRv2SP6	5′-tagcaatttaggtgacactatagaaacttcttctgtcctttgtcgttct-3′
H4		H4RNAiFwd1T7	5′-gatcataatacgactcactatagggatgtctggacgcggaaaag-3′
		H4RNAiRv1SP6	5′-tagcaatttaggtgacactatagaactttagtacacctctggtttcctc-3′
		H4RNAiFwd2T7	5′-gatcataatacgactcactataggggtcaaacgtatctctggccttat-3′
		H4RNAiRv2SP6	5′-tagcaatttaggtgacactatagaaaacctccgaatccgtaaagag-3′
		cMycInsituRVsp6	5′-agtgcatttaggtgacactatagaattgttaacggaaaagggaaaactg-3′
Plasmid		gfpRv-SAC1	5′-aaaaagagctcctatttgtatagttcatccatgccatg-3′
		TerminatorFw-PacI	5′-aaaaattaattaacgtacgggccctttcgtct-3′
Race		NewsplicedleaderFwd	5′-tactcacactatttctaagtccctgagtttaag-3′
		PiwiRV2SP6	5′-tagcaatttaggtgacactatagaaccttagcgccacctgtgc-3′
Cloning		LigDVectorGFP-Fusion	5′-gcggccgctgcagccccggt-3′
		BackbonelactRV1	5′-actggccgtcgttttacaac-3′
		Piwi1ProFw1new	5′-cagatgatccgcagacaatagac-3′
		Piwipromrevin	5′-gttttcttcttataatttttctaaaaactt-3′
		PiwiRv2SP6	5′-tagcaatttaggtgacactatagaaccttagcgccacctgtgc-3′
		Piwi1TerRV1-PacI	5′-aaaaattaattaagaaggcttacgctagtgtgaattag-3′
		Piwi1TerFw1-SAC1	5′-aaaaagagctcgtagctgcgcgttgtttacg-3′
		GFPSeqFusRev	5′-ttgcatcaccttcaccctctcc-3′
		PBIGFor	5′-taaaaataggcgtatcacgaggccc-3′