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. 2015 May 6;5:10024. doi: 10.1038/srep10024

Figure 2. Effect of CagA expression on NF-κB signaling in mice.

Figure 2

(a) Immunoblot analysis of IκBα in the colon of 48-week-old cagA-Tg and control mice. Lysates prepared from the colon were immunoblotted with the indicated antibodies (left panel). Quantification of the intensity of IκBα relative to actin (right panel). Error bars, mean ± s.d. (n = 3). ***P < 0.001 (Student’s t-test). (b) Immunostaining of IκBα in the colonic mucosa from cagA-Tg mice or control mice without DSS treatment. Scale bars, 50 μm (c) Immunoblot analysis of phosphorylated p65 (p-p65) in the colon of 48-week-old cagA-Tg and control mice (left panel). Quantification of the intensity of p-p65 relative to actin (right panel). Error bars, mean ± s.d. (n = 3). (d) Immunostaining of p65 (upper panel) and phosphorylated IKKα/β (pIKK) (lower panel) in the colonic mucosa of 48-week-old cagA-Tg or control mice. Nuclei were visualized by DAPI. Scale bars, 10 μm. (e) Immunoblot analysis of IκBα in the stomach of 48-week-old cagA-Tg and control mice (left panel). Quantification of the intensity of IκBα relative to actin (right panel). Error bars, mean ± s.d. (n = 3). ***P < 0.001 (Student’s t-test). (f) Immunostaining of IκBα in the stomach from cagA-Tg mice or control mice without DSS treatment. Scale bars, 100 μm. (g) Immunostaining of p65 in the stomach from cagA-Tg mice or control mice without DSS treatment. Nuclei were visualized by DAPI. Scale bar, 10 μm. (h) Immunostaining of p65 in the colonic mucosa from cagA-Tg mice or control mice with DSS treatment. Scale bar, 10 μm (upper panel). Percentage of cells showing nuclear localization of p65 (lower panel). Error bars, mean ± s.d. (n = 6). ***P < 0.001 (Student’s t-test).