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. 2015 May 1;29(9):898–903. doi: 10.1101/gad.261743.115

Figure 4.

Figure 4.

Deletion of one copy of DSGs from disomic yeast strains does not improve cellular fitness. (A) Cells harboring an additional copy of chromosome V (A28265) or an additional copy of chromosome V lacking GLN3 (A36470) were grown as described in Figure 1 to measure their doubling time. (B) Cells harboring an additional copy of chromosome XVI (A34149) or an additional copy of chromosome XVI lacking the indicated genes (A36585, A36650, A36689, and A36859) were grown as described in Figure 1 to measure their doubling time. (C, left graph) Cells with an additional copy of chromosome IX (A13975) or an additional copy of chromosome IX lacking the indicated genes (A36233, A36215, and A36069) were grown as described in Figure 1 to measure their doubling time. The middle and right graphs show competition experiments in which equal numbers of disome IX cells harboring GFP (A36219) and disome IX cells lacking the indicated genes (A36233 and A36215) were coinoculated in –His G418 medium. The percentages of GFP-positive and GFP-negative cells were monitored at the indicated times. (D, left graph) Cells with an additional copy of chromosome XI (A28266) or an additional copy of chromosome XI lacking the indicated genes (A36919 and A36336) were grown as described in Figure 1 to measure their doubling time. The middle and right graphs show competition experiments in which equal numbers of disome XI cells expressing GFP (A36222) and GFP− disome XI cells lacking the indicated genes (A36919 and A36336) were coinoculated in −His G418 medium. The percentages of GFP-positive and GFP-negative cells were monitored at the indicated times.