Figure 1.

Experimental design flow-chart. (A) B73 seedlings (all derived from a single self-pollination) were grown for 14 days. Some of these plants were then subjected to cold, heat, or UV-B stress for 4 h per day (every other day for four treatments). At the conclusion of the treatment regime the stressed plants were then grown under standard conditions to maturity. DNA was isolated from the flag leaf (the last adult leaf initiated before the tassel) and used to perform meDIP-chip. (B) Tissue culture samples from the genotype A188 was used to perform regeneration. The plantlets were then grown to maturity (along with sibling non-tissue culture A188 plants) and tissue was collected from the flag leaf for meDIP-chip.