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. 2015 May 6;6:312. doi: 10.3389/fpls.2015.00312

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Copyright © 2015 Li, Pu, Feng, Yang, Shen, Zhang and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of the FocVel1 deletion strategy. (A) FocVel1 and hygromycin-resistance (hph) cassettes are denoted by large gray and white arrows, respectively. Red arrows represent the restriction enzyme sites of XhoI. (B) Southern blot hybridization analysis. M, λ-EcoT14 I digest; lane 1, genomic DNA preparations of the wildtype strain (Foc-GD); lane 2, the FocVel1 deletion mutant (ΔFocVel1-3); lane 3, the complement strain (ΔFocVel1-3C).