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. 2015 May 6;6:134. doi: 10.3389/fphys.2015.00134

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Copyright © 2015 Chen, Zhang, Guo and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In situ confocal imaging of T-tubules on intact rat heart and T-tubule image analysis. (A) A typical t-tubule image from the epicardium of Langendorff-perfused intact rat heart (control, sham-operated) loaded with lipophilic membrane indicator FM4-64. (B) The periodically organized T-tubules were viewed from a 3D reconstruction of 25 confocal stacks at 0.2 μm interval. Scale bar: 20 μm. (C) Power spectrum retrieved from a 2D Fourier transformation of T-tubule image characterized the power magnitude of the regular organization of T-tubule system. Data are adapted from Wei et al. (2010).