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. 2015 May 6;6:406. doi: 10.3389/fmicb.2015.00406

FIGURE 3.

FIGURE 3

Genome organization of SsBRV1. (A) Northern blot analysis of SsBRV1 dsRNA1 and dsRNA2. dsRNA from viral particles were subjected to Northern blot analysis. The positions of probes 1 to 3 are shown in panel B. (B) Schematic representation of the genetic organization of dsRNA1 and dsRNA2 segments of the SsBRV1, and genome segment assignment of the structural proteins. dsRNA1 and 2 are 6,457 and 5,965 nt in length, respectively. dsRNA1 has a 568-nt-long 5′ UTR, one ORF (ORF1), and a 114-nt-long 3′ UTR, whereas dsRNA2 has a 577-nt-long 5′ UTR, one ORF(ORF2), and a 117-nt-long 3′ UTR. ORF1 and two are composed of 1,925 and 1,757 codons, respectively. Open boxes drawn with solid lines denote the ORFs. The gray position on ORF1 and 2 shows the conserved domain RdRp domain (RdRp_4, Pfam 02123). Polypeptides with sizes of approximately 120, 100, and 80 kDa corresponding to the region of the ORF1- and ORF2-encoded polyproteins are colored in light green and blue, respectively. The dashed line indicates the region of tryptic peptides identified by MS analysis (see Supplementary Table S3). The growth hormone receptor (GHR) binding domain (GHBP, Pfam 12772) in ORF2-encoded polyprotein is also indicated. The gray area between dsRNA1 and 2 indicates the conserved domain with significant similarity at protein level. The numbers above solid lines refer to the map positions of the initiation and termination codons of the respective ORF.