Figure 4.

A conceptual representation of the T₅₀ test. CPP, calciprotein particle; T₅₀, lag time. Protocol adapted from Pasch et al.101. To form a precipitation mix, Ca and Pi stock solution was added to serum of a healthy volunteer (right) and a dialysis patient (left) and incubated at 37°C. A standard flash enabled camera was used to capture the images obtained at the given time intervals. CPP1 stage appears translucent due to a relatively small degree of light scattering. After 255 minutes, the dialysis patient sample becomes opaque, indicating that CPP1 has transformed into CPP2, but the sample from the healthy volunteer remained translucent until 400 min, when it underwent a similar change in opacity.