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. 2015 May 6;4:690. doi: 10.1038/bonekey.2015.58

Figure 1.

Figure 1

Innervation of CGRP-positive sensory neurons in the bone. Bones were fixed, decalcified and cut on a cryostat (30 μm). Sections were then incubated with a guinea pig anti-CGRP (1:2000), followed by an incubation with goat anti-guinea pig IgG (1:500). The immunostained sections were examined under a confocal laser-scanning microscope (LSM510, Ver 3.2, Carl Zeiss). CGRP-positive sensory neurons (black) run through mineralized bone into the bone marrow. CGRP, calcitonin gene-related peptide.