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. 2015 May 6;6:211. doi: 10.3389/fimmu.2015.00211

Figure 2.

Figure 2

Correlation between levels of anti-A/B antibodies as detected by surface plasmon resonance using the Biacore® device and levels of B cell subsets in CVID patients. Anti-A/B antibodies were determined using surface plasmon resonance (SPR) technology with synthetic blood group A/B trisaccharides coupled to the CM-5 Biacore® chip by amine binding, antibody titers are expressed as resonance units (RU). Peripheral B cell subsets were determined by three-color-flow cytometry, results are depicted as the percentage of total number of CD19+ B lymphocytes. Horizontal lines indicate the normal range of B cell subsets as determined in 134 healthy individuals (upper panel: 5th percentile; middle and lower panel: upper line, 95th percentile, lower line, 5th percentile). The Bravais–Pearson-correlation coefficient was calculated to determine a statistically significant correlation between two parameters at a level ofP < 0.05.