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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Neuroepigenetics. 2015 Apr 1;2:1–8. doi: 10.1016/j.nepig.2015.03.001

Figure 1.

Figure 1

(a) Graphic depicting the 8 day protocol used in Experiment 1. For 8 days rats performed a foraging task in a familiar environment (Environment A). On days 4–8, rats performed the task in two 20 lap sessions separated by 10 minutes. On days 1–7, protocols were identical for the experimental and control group. On day 8 the second 20 lap session for the experimental group was conducted in a novel environment (Environment B). Brains were extracted for biochemical analyses 90 minutes after the second foraging session on day 8. (b) Experience-induced alterations of hippocampal Bdnf gene DNA methylation. Levels of methylated and unmethylated DNA associated with the Bdnf gene in dentate (Bdnf I n=10, Bdnf IV n=11), CA3 (Bdnf I n=10, Bdnf IV n=11, and CA1 (Bdnf I n=11, Bdnf IV n=11) in novel-exposed vs. control (familiar-exposed) rats. (c) Methylation analysis of individual CG dinucleotides associated with exon I in the dentate of control and novel-exposed rats (n=10/group). (d) Bdnf mRNA (exon IX) levels in novel-exposed rats relative to familiar-exposed controls (DG n=8, CA3 n=9, CA1 n=9). Error bars represent SEM; *p<0.05; ++p=0.0885.