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. 2015 Apr 29;5(4):150011. doi: 10.1098/rsob.150011

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© 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — Effect of low-dose IR upon cell proliferation in the mouse lens epithelium. (a) Cell densities were measured in the peripheral region, for area 1 and area 2, 24 h after exposure to the indicated IR doses. Areas 1 and 2 are consecutive fields of view, separated by a few pixels, of the lens periphery. Both dose and area were significant factors (GLM ANOVA, p both <0.001). There was no significant interaction detected (area × dose p = 0.066). Dunnett's test for comparison with a control revealed that 250 and 1000 mGy both produced statistically significantly higher densities for area 1 (p = 0.002 and 0.007, respectively) and 100 and 250 mGy were significantly higher in area 2 (p = 0.042 and 0.007, respectively); by contrast, 50, 100 and 2000 mGy were statistically indistinguishable from the control (p all >0.05). (b) Cell proliferation was measured by EdU incorporation. Both dose and area were significant (GLM ANOVA, p both <0.001). There was a significant interaction between area and dose (p < 0.001); Dunnett's test for comparison with a control revealed that 100 and 250 mGy produced significantly higher EdU labelling (area 1, p both <0.001), a trend that was also observed in area 2, but with small differences between the labelling (p = 0.027 and <0.001, respectively). TUNEL staining showed no increase after IR exposure (data not shown). (c) Effects of IR upon cyclin D1 levels in the peripheral region of the lens. In controls, area 1 contains cells that are cyclin D1 positive, while area 2 does not. IR increased the cyclin D1 signal in area 2 for 100 and 250 mGy levels. Both dose and area were significant (GLM ANOVA, p both <0.001). A significant interaction between area and dose (p < 0.001) was observed. Dunnett's test for comparison with a control revealed that 100 and 250 mGy produced significantly increased cyclin D1 for area 1 (p < 0.001 and 0.005, respectively), whereas 1000 and 2000 significantly decreased the cell proliferation (p both <0.001); 50 mGy was not significantly different from the control. For area 2, only the 100 and 250 mGy points showed significantly higher levels of cyclin D1 (p < 0.001 in both cases); 50, 1000 and 2000 mGy were indistinguishable from the control (p all > 0.999). Vertical arrows, 320 µm. Scale bars, 25 µm.