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. 2015 Apr 22;5(4):150030. doi: 10.1098/rsob.150030

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© 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — NG peptides are ligands for the S. purpuratus NPS/CCAP-type receptor. Dose–response curves for Ca²⁺ responses evoked by the NG peptides NGFFFamide (S. purpuratus), NGFFYamide (A. rubens) and NGIWYamide (A. japonicus) in CHO-K1 cells expressing the S. purpuratus NPS/CCAP-type receptor. Each point (±s.e.m.) represents mean values from at least two independent experiments performed in at least triplicate. Dose–response data are shown as relative (%) to the highest value (100% activation) after normalization to the maximum Ca²⁺ response. The log EC₅₀ values (±s.e.m.) are NGFFFamide: −9.38 ± 0.09; NGFFYamide: −8.25 ± 0.04; NGIWYamide: −6.44 ± 0.04.